5WG7
Human Carbonic Anhydrase II complexed with AceK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9782 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.312, 41.067, 72.152 |
Unit cell angles | 90.00, 103.84, 90.00 |
Refinement procedure
Resolution | 41.083 - 1.450 |
R-factor | 0.1434 |
Rwork | 0.142 |
R-free | 0.17610 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.858 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.10pre_2097) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.100 |
High resolution limit [Å] | 1.450 |
Number of reflections | 63242 |
<I/σ(I)> | 12.9 |
Completeness [%] | 96.7 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | 1.6 M sodium citrate and 50 mM Tris |