5WG7

Human Carbonic Anhydrase II complexed with AceK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9782
Spacegroup name	P 1 21 1
Unit cell lengths	42.312, 41.067, 72.152
Unit cell angles	90.00, 103.84, 90.00

Refinement procedure

Resolution	41.083 - 1.450
R-factor	0.1434
Rwork	0.142
R-free	0.17610
RMSD bond length	0.005
RMSD bond angle	0.858
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10pre_2097)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.100
High resolution limit [Å]	1.450
Number of reflections	63242
<I/σ(I)>	12.9
Completeness [%]	96.7
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	298	1.6 M sodium citrate and 50 mM Tris