5WFV
Kelch domain of human Keap1 bound to Nrf2 ETGE peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.919, 69.253, 79.228 |
Unit cell angles | 90.00, 118.50, 90.00 |
Refinement procedure
Resolution | 52.140 - 1.910 |
R-factor | 0.2002 |
Rwork | 0.200 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wlf |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.140 | 1.978 |
High resolution limit [Å] | 1.690 | 1.910 |
Rmerge | 0.069 | 0.330 |
Number of reflections | 58943 | 5843 |
<I/σ(I)> | 15.18 | 3.74 |
Completeness [%] | 93.0 | 97 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.994 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 1.2 - 1.5 M Ammonium Sulfate, 0.1 M Bis-Tris pH = 6.0 - 6.5 |