5WF0
70S ribosome-EF-Tu H84A complex with GTP and near-cognate tRNA (Complex C2)
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.26 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.6 |
Number of Particle | 193521 |
Software | RELION,REFMAC,Leginon,Gctf,Coot |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |