5WE0

Structural Basis for Shelterin Bridge Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.999996
Spacegroup name	P 1
Unit cell lengths	56.720, 82.010, 103.510
Unit cell angles	89.99, 89.98, 73.96

Refinement procedure

Resolution	62.705 - 2.300
R-factor	0.1994
Rwork	0.199
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.123
Phasing software	PHENIX ((1.11.1_2575: ???))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	62.710
High resolution limit [Å]	2.300
Number of reflections	77768
<I/σ(I)>	5.4
Completeness [%]	97.6
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Sodium citrate tribasic dihydrate, 21% w/v PEG3350