5WDP

H-Ras mutant L120A bound to GMP-PNP at 277K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	277
Detector technology	PIXEL
Collection date	2017-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88557
Spacegroup name	H 3 2
Unit cell lengths	89.988, 89.988, 135.969
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.323 - 1.350
R-factor	0.1455
Rwork	0.145
R-free	0.15360
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3k8y
RMSD bond length	0.009
RMSD bond angle	1.052
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.320	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.099	2.972
Rpim	0.023	0.678
Number of reflections	46642	2277
<I/σ(I)>	16.2	1.2
Completeness [%]	100.0	100
Redundancy	19.7	20
CC(1/2)	0.999	0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	17% PEG8000, 200 mM calcium acetate, 100 mM MES/NaOH, pH 6.0