5WDL

A processive dipeptidyl aminopeptidase secreted from an established commensal bacterium P. distasonis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	104.841, 140.299, 221.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.105 - 2.625
R-factor	0.1729
Rwork	0.171
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pw3
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.150	1.721
Rpim	0.071	0.498
Number of reflections	96577	4696
<I/σ(I)>	12.7	1.7
Completeness [%]	99.6	98.5
Redundancy	5.9	5.1
CC(1/2)		0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.27 M K/Na Tartrate, 22% PEG3350, 1 mM ZnCl