5WDD
Crystal structure of chicken BOK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.559, 59.620, 64.083 |
| Unit cell angles | 90.00, 96.26, 90.00 |
Refinement procedure
| Resolution | 36.526 - 1.799 |
| R-factor | 0.1973 |
| Rwork | 0.195 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bd8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.653 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.530 | 1.863 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Rmerge | 0.066 | 2.155 |
| Rpim | 0.037 | 1.255 |
| Number of reflections | 28655 | 2803 |
| <I/σ(I)> | 12.93 | 0.8 |
| Completeness [%] | 98.5 | 95.75 |
| Redundancy | 3.9 | 3.7 |
| CC(1/2) | 0.999 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 22.5% PEG 3350, 90 mM trisodium citrate pH 3.5, 3% ethylene glycol |
