5WCG

SET and MYND Domain Containing protein 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97929
Spacegroup name	C 1 2 1
Unit cell lengths	92.343, 98.312, 58.833
Unit cell angles	90.00, 101.95, 90.00

Refinement procedure

Resolution	50.000 - 2.020
R-factor	0.1708
Rwork	0.170
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s7b
RMSD bond length	0.010
RMSD bond angle	1.402
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	2.020	5.480	2.020
Rmerge	0.145	0.055	0.984
Rmeas	0.155	0.061
Rpim	0.071	0.027	0.514
Number of reflections	33305		1647
<I/σ(I)>	6.5
Completeness [%]	100.0	99.7	100
Redundancy	4.9	4.8	4.5
CC(1/2)		0.998	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	20% PEG 3350, 0.04M Citric acid, 0.06M Bis-Tris propane pH6.4