5WCF

Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	I 41
Unit cell lengths	94.721, 94.721, 108.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.980
R-factor	0.1999
Rwork	0.200
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hc4
RMSD bond length	0.009
RMSD bond angle	1.372
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.060	0.033	0.907
Rmeas	0.065	0.035	0.973
Rpim	0.024	0.013	0.351
Total number of observations	253023
Number of reflections	33057		1649
<I/σ(I)>	13
Completeness [%]	100.0	99.4	100
Redundancy	7.7	7.5	7.7
CC(1/2)		0.999	0.758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	15% PEG 8000, 0.2M MgCl2, 0.1M Tris-HCl pH8.5