5WBW

Yeast Hsp104 fragment 1-360

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2001-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0396
Spacegroup name	P 21 21 21
Unit cell lengths	66.556, 75.824, 235.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.043 - 2.600
R-factor	0.2408
Rwork	0.237
R-free	0.28590
RMSD bond length	0.002
RMSD bond angle	0.558
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.680
High resolution limit [Å]	2.590	2.590
Number of reflections	36873	2973
<I/σ(I)>	16.5
Completeness [%]	97.3	80.3
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	25% PEG4000, 50 mM Tris-HCl, 20 mM ammonium citrate