5WBW
Yeast Hsp104 fragment 1-360
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2001-03-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0396 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.556, 75.824, 235.649 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.043 - 2.600 |
R-factor | 0.2408 |
Rwork | 0.237 |
R-free | 0.28590 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.558 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.040 | 2.680 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 36873 | 2973 |
<I/σ(I)> | 16.5 | |
Completeness [%] | 97.3 | 80.3 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 25% PEG4000, 50 mM Tris-HCl, 20 mM ammonium citrate |