5WB9
Crystal structure of CD4 binding site antibody N60P23 in complex with HIV-1 clade A/E strain 93TH057 gp120 core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-06 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.562, 68.567, 119.356 |
| Unit cell angles | 90.00, 111.36, 90.00 |
Refinement procedure
| Resolution | 37.330 - 2.400 |
| R-factor | 0.21574 |
| Rwork | 0.213 |
| R-free | 0.25763 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.160 | 0.908 |
| Number of reflections | 38617 | |
| <I/σ(I)> | 9.7 | 1.2 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M MOPS pH 7.5, ).1M Magnesium acetate hexahydrate |
