5WA1

CHMP4C A232T in complex with ALIX BRO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	120.979, 61.450, 76.187
Unit cell angles	90.00, 121.70, 90.00

Refinement procedure

Resolution	37.811 - 1.868
R-factor	0.1946
Rwork	0.194
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3c3r
RMSD bond length	0.005
RMSD bond angle	0.717
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.811	1.935
High resolution limit [Å]	1.868	1.868
Rmerge	0.022	0.808
Number of reflections	38802	3744
<I/σ(I)>	8.78	0.97
Completeness [%]	97.6	93.38
Redundancy	2
CC(1/2)	0.999	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	15% PEG8000, 100 mM MES, pH 6.5, 200 mM sodium acetate