5W7W

Crystal Structure of FHA domain of human APLF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-08
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.514
Spacegroup name	P 21 21 2
Unit cell lengths	56.794, 96.944, 34.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.267 - 1.348
R-factor	0.148
Rwork	0.147
R-free	0.16420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2brf
RMSD bond length	0.007
RMSD bond angle	0.944
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.267	1.370
High resolution limit [Å]	1.348	1.348
Rpim	0.027	0.245
Number of reflections	41939	1696
<I/σ(I)>	24.8
Completeness [%]	97.2	80
Redundancy	7	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.5M sodium formate