5W74
Crystal Structure of the Group II Chaperonin from Methanococcus Maripaludis D386ADeltaLid Mutant in the Open, ADP-Bound State
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 152.720, 221.930, 262.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.589 - 3.650 |
R-factor | 0.2653 |
Rwork | 0.265 |
R-free | 0.27460 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.531 |
Data reduction software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.589 | 3.710 |
High resolution limit [Å] | 3.650 | 3.650 |
Number of reflections | 96789 | 4255 |
<I/σ(I)> | 11.3 | 1.5 |
Completeness [%] | 97.4 | 88.7 |
Redundancy | 15.5 | 11.5 |
CC(1/2) | 0.997 | 0.329 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M MES pH 6.5, 5mM spermi- dine, 30% 2-Methyl-2,4-pentanediol |