5W6W
Designing Higher Resolution Self-Assembled 3D DNA Crystals via Strand Terminus Modifications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | H 3 |
Unit cell lengths | 106.712, 106.712, 94.081 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.960 - 3.060 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbi |
RMSD bond length | 0.016 |
RMSD bond angle | 1.451 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.107 | 0.431 |
Number of reflections | 5152 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 68.0 | 19.2 |
Redundancy | 7.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Grown by vapor diffusion while treated with a controlled temperature gradient from 333K degs to 293K. They were grown in Ammonium Sulfate, TRIS, Acetic Acid and EDTA. |