5W6L
Crystal Structure of RRSP, a MARTX Toxin Effector Domain from Vibrio vulnificus CMCP6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 3 |
Unit cell lengths | 247.103, 247.103, 247.103 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 3.450 |
R-factor | 0.22888 |
Rwork | 0.228 |
R-free | 0.24913 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.012 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.510 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.113 | 0.866 |
Rmeas | 0.046 | 0.382 |
Number of reflections | 33078 | 1638 |
<I/σ(I)> | 18.4 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 6.8 | 5.9 |
CC(1/2) | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Protein: 15.6 mg/ml, 0.5M Sodium chloride, 0.01M Tris HCl (pH 8.3); Screen: 1.96M Ammonium sulfate, 0.1 MOPS (pH 6.5), 20% (v/v) PEG400; Cryo: 4M Formate |