5W6D

Crystal structure of BG505-SOSIP.v4.1-GT1-N137A in complex with Fabs 35022 and 9H/109L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03322
Spacegroup name	P 63
Unit cell lengths	126.952, 126.952, 315.199
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.433 - 3.202
R-factor	0.2437
Rwork	0.242
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.433	3.260
High resolution limit [Å]	3.200	3.200
Rpim	0.077	0.770
Number of reflections	47220	2375
<I/σ(I)>	11	1
Completeness [%]	100.0	100
Redundancy	12.9	13.1
CC(1/2)	0.890	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	277	0.2M NaCl, 34% PEG 300, 0.1M NaOAc