5W66

RNA polymerase I Initial Transcribing Complex State 3

EM specimen

Vitrificationfalse
Stainingfalse

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.9
Software,Leginon,Gctf,NAMD,UCSF Chimera,Coot,PHENIX,RELION
SymmetryC1
CTF correctionPHASE FLIPPING ONLY