5W3V

Crystal Structure of macaque APOBEC3H in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.283
Spacegroup name	P 2 21 21
Unit cell lengths	87.960, 89.310, 134.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.655 - 2.243
R-factor	0.1839
Rwork	0.182
R-free	0.22150
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800
High resolution limit [Å]	2.590
Rmerge	0.086	1.080
Number of reflections	69877
<I/σ(I)>	15.7
Completeness [%]	100.0
Redundancy	7.1
CC(1/2)	1.000	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1M Tris:HCl pH 8.5 0.1M MgCl2 28% PEG 4000
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	0.1M Tris:HCl pH 8.5 0.1M MgCl2 28% PEG 4000