5W3P
ANTIBODY C706 IN COMPLEX WTH BETA-AMYLOID PEPTIDE 1-16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.200, 69.880, 104.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.920 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.068 | 0.188 |
| Number of reflections | 34530 | 1542 |
| <I/σ(I)> | 36.5 | 9.7 |
| Completeness [%] | 92.3 | 56.7 |
| Redundancy | 17.1 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2.0 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, PH 4.5 |
