5W36
Crystal structure of the RNA polymerase domain (RPD) of Mycobacterium tuberculosis primase DnaG in complex with a double-stranded DNA oligomer with a 6-nucleotide overhang
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-25 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.837, 210.936, 46.046 |
| Unit cell angles | 90.00, 106.78, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.460 |
| R-factor | 0.21775 |
| Rwork | 0.215 |
| R-free | 0.26915 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w34 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.450 |
| Number of reflections | 27849 |
| <I/σ(I)> | 23 |
| Completeness [%] | 96.2 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 100 mM sodium citrate pH 6.0, 5% w/v PEG 4000 and 5 mM SrCl2 |
