5W2J
Crystal structure of dimeric form of mouse Glutaminase C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2011-10-12 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.940, 100.880, 145.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.500 |
Rwork | 0.196 |
R-free | 0.22761 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.890 | |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 51086 | |
<I/σ(I)> | 50.9 | |
Completeness [%] | 92.9 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.5 | 293.15 | 9% PEG8000, 10% glycerol, 50 mM Tris-HCl, pH 8.5 |