5W1J

Echinococcus granulosus thioredoxin glutathione reductas (egTGR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	108.740, 108.740, 257.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.783 - 2.700
R-factor	0.2001
Rwork	0.197
R-free	0.25970
RMSD bond length	0.013
RMSD bond angle	1.605
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Rmerge	0.062
Number of reflections	36071
<I/σ(I)>	14.9
Completeness [%]	99.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	18% PEG3350, 0.1 M lithium sulfate, 0.1 M Tris-HCl, pH7.5