5W1H
Crystal structure of LbaCas13a (C2c2) bound to mature crRNA (24-nt spacer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.842, 92.213, 115.858 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 47.120 - 1.990 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.482 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.11.1-2575-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.079 | 0.841 |
| Number of reflections | 107265 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 98.8 | 89.7 |
| Redundancy | 6.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M ammonium iodide, 20 - 25 % PEG 3,350 |
