5VZU
Crystal structure of the Skp1-FBXO31-cyclin D1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.679, 156.235, 155.522 |
Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.700 |
R-factor | 0.1829 |
Rwork | 0.180 |
R-free | 0.23300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vzt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.445 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
Rmerge | 0.059 | 0.027 | 0.696 |
Total number of observations | 416593 | ||
Number of reflections | 55014 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 99.1 | 98.7 | 90.9 |
Redundancy | 7.6 | 7.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 100 mM sodium citrate (pH 5.3), 0.27 M ammonium acetate, 13-17% MPD, 0.02 M CaCl2 |