5VZU

Crystal structure of the Skp1-FBXO31-cyclin D1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	C 1 2 1
Unit cell lengths	87.679, 156.235, 155.522
Unit cell angles	90.00, 104.21, 90.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.1829
Rwork	0.180
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vzt
RMSD bond length	0.011
RMSD bond angle	1.445
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.059	0.027	0.696
Total number of observations	416593
Number of reflections	55014
<I/σ(I)>	9.7
Completeness [%]	99.1	98.7	90.9
Redundancy	7.6	7.6	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	277	100 mM sodium citrate (pH 5.3), 0.27 M ammonium acetate, 13-17% MPD, 0.02 M CaCl2