5VZI

Post-catalytic complex of human Polymerase Mu (W434H) mutant with incoming dTTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	60.132, 68.838, 111.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.875 - 1.500
R-factor	0.1722
Rwork	0.171
R-free	0.19360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.024	0.249
Number of reflections	73085	3020
<I/σ(I)>	26.72	3
Completeness [%]	97.9	82.7
Redundancy	7	4.9
CC(1/2)		0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	95mM HEPES, 19% PEG4K