5VYG
Crystal structure of hFA9 EGF repeat with O-glucose trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-06 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.417, 46.840, 37.259 |
| Unit cell angles | 90.00, 92.58, 90.00 |
Refinement procedure
| Resolution | 46.160 - 2.200 |
| R-factor | 0.21448 |
| Rwork | 0.212 |
| R-free | 0.25895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.104 | 0.515 |
| Number of reflections | 7814 | 1200 |
| <I/σ(I)> | 12.6 | 4.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Tris pH 8.5, 15% Glycerol, 1.6 M (NH4)2SO4, 5% 2,2,2-Trifluoroethanol |
