5VYD

Crystal structure of phosphodiesterase domain of RhoPDE fusion protein from the Choanoflagellate Salpingoeca rosetta

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	74.649, 96.400, 108.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.986 - 2.300
R-factor	0.2177
Rwork	0.216
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1taz
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX (dev_2719)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.129	0.794
Number of reflections	34788	4804
<I/σ(I)>	12.9	2.8
Completeness [%]	98.0	94.8
Redundancy	12.2	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	15% PEG3350, 100 mM succinic acid