5VYC
Crystal structure of the human 40S ribosomal subunit in complex with DENR-MCT-1.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 334.670, 597.130, 336.500 |
| Unit cell angles | 90.00, 120.29, 90.00 |
Refinement procedure
| Resolution | 116.931 - 6.000 |
| R-factor | 0.3227 |
| Rwork | 0.323 |
| R-free | 0.32070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 5A2Q (40S subunit only) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 120.000 |
| High resolution limit [Å] | 6.000 |
| Number of reflections | 282736 |
| <I/σ(I)> | 5.5 |
| Completeness [%] | 99.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 298.15 | 50 mM HEPES-NaOH, 50 mM NH4 acetate, 50 mM NH4Cl, 1 mM Mg acetate, 2 mM CaCl2, 3% PEG 20000, 3% PEG 3350, 2 mM TCEP, pH 8.1 |
