5VXR
The antigen-binding fragment of MAb24 in complex with a peptide from Hepatitis C Virus E2 epitope I (412-423)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-19 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 70.370, 53.338, 133.903 |
Unit cell angles | 90.00, 93.53, 90.00 |
Refinement procedure
Resolution | 14.110 - 1.400 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gag |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | XDS (20151015) |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER (2.6.0) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.480 | 42.480 | 1.390 |
High resolution limit [Å] | 1.360 | 7.460 | 1.360 |
Rmerge | 0.050 | 0.036 | 3.069 |
Rmeas | 0.054 | 0.039 | 3.679 |
Rpim | 0.019 | 0.014 | 1.985 |
Number of reflections | 67898 | ||
<I/σ(I)> | 32.9 | ||
Completeness [%] | 64.2 | 95.1 | 4.2 |
Redundancy | 8 | 7 | 2.9 |
CC(1/2) | 0.999 | 0.998 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 12.5% PEG3000, 100 mM sodium chloride, 100 mM sodium phosphate dibasic/citric acid, pH 6.2 |