5VXC
Crystal Structure Analysis of human CLYBL in complex with free CoASH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-09-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99997 |
Spacegroup name | H 3 |
Unit cell lengths | 76.515, 76.515, 139.428 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 59.849 - 1.872 |
R-factor | 0.1832 |
Rwork | 0.181 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z6k |
RMSD bond length | 0.010 |
RMSD bond angle | 0.862 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.849 | 1.878 |
High resolution limit [Å] | 1.872 | 1.872 |
Rmerge | 0.087 | 0.802 |
Rmeas | 0.097 | 0.896 |
Rpim | 0.042 | 0.394 |
Total number of observations | 137154 | |
Number of reflections | 24985 | |
<I/σ(I)> | 15.7 | |
Completeness [%] | 99.5 | 100 |
Redundancy | 5.5 | 5.3 |
CC(1/2) | 0.998 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 1 uL protein at 4 mg/ml and well solution (0.1 M sodium acetate:HCl pH4.6, 3.5 M sodium formate) |