5VWX

Bak core latch dimer in complex with Bim-h0-h3Glt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2017-03-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9464
Spacegroup name	P 21 21 21
Unit cell lengths	61.629, 76.481, 77.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.248 - 2.489
R-factor	0.2557
Rwork	0.250
R-free	0.30470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.416
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250
High resolution limit [Å]	2.489	2.489
Rmerge	0.165
Number of reflections	13253
<I/σ(I)>	8.51
Completeness [%]	98.0
Redundancy	7.3
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	281	200 mM calcium acetate, 30 % PEG 400 and 100 mM sodium acetate-acetic acid pH 5.0