5VWS
Ligand free structure of Cytochrome P450 TbtJ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 95.316, 95.316, 126.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.221 - 2.411 |
R-factor | 0.212 |
Rwork | 0.208 |
R-free | 0.28080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wio |
RMSD bond length | 0.009 |
RMSD bond angle | 1.020 |
Data reduction software | KYLIN |
Data scaling software | KYLIN |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.221 |
High resolution limit [Å] | 2.410 |
Rmerge | 0.010 |
Number of reflections | 21196 |
<I/σ(I)> | 6 |
Completeness [%] | 91.4 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 1 M (NH4)2SO4 1 % PEG 3350 0.1 M Bis-Tris pH 5.5 |