5VVR
Ternary complex of RNA Pol II, transcription scaffold and Rad26
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 5.8 |
| Software | RELION,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
