5VTD

Crystal Structure of the Co-bound Human Heavy-Chain Ferritin variant 122H-delta C-star

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-06
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	180.244, 180.244, 180.244
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.720 - 1.950
R-factor	0.178
Rwork	0.174
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cmq
Data reduction software	SAINT
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.720	2.030
High resolution limit [Å]	1.950	1.950
Rmerge	0.132	0.607
Number of reflections	18852
<I/σ(I)>	10.9	2
Completeness [%]	99.5	98.7
Redundancy	8.1	4.2
CC(1/2)	0.991	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Reservoir: 500 uL total volume: 25 mM Tris (pH 8), 12 mM CaCl2, 150 mM NaCl, 0.3 mM CoCl2, 1% PEG 1900 MME Sitting Drop: 2 uL reservoir, 2 uL of 4 uM ferritin