5VSV

Crystal Structure of Inosine 5'-monophosphate Dehydrogenase from Clostridium perfringens Complexed with IMP and P225

Replaces:  5UZO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-27
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.79719
Spacegroup name	P 1
Unit cell lengths	62.861, 77.637, 78.972
Unit cell angles	110.83, 104.05, 105.44

Refinement procedure

Resolution	37.133 - 2.205
R-factor	0.2
Rwork	0.198
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 4Q32
RMSD bond length	0.007
RMSD bond angle	0.956
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	59509	2929
<I/σ(I)>	6.3	1.6
Completeness [%]	96.3	94.1
Redundancy	2	1.8
CC(1/2)		0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	289	17.1% PEG600, 50mM Bicine pH 8.4, 4.3% PEG2000MME