5VSU
Structure of yeast U6 snRNP with 2'-phosphate terminated U6 RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.157, 114.728, 179.844 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.723 - 3.100 |
R-factor | 0.2386 |
Rwork | 0.234 |
R-free | 0.29820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.667 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.723 | 3.290 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.250 | 4.031 |
Number of reflections | 27164 | |
<I/σ(I)> | 13 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 51.2 | 47.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 0.2 M NH4F 0.1 M HEPES pH 7.4 0.01 M MgCl2 18 % PEG 3,350 |