5VSM
Crystal structure of viperin with bound [4Fe-4S] cluster, 5'-deoxyadenosine, and L-methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.364, 73.264, 141.939 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.534 - 1.700 |
R-factor | 0.1603 |
Rwork | 0.159 |
R-free | 0.19460 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | Viperin with bound [4Fe-4S] cluster and S-adenosylhomocysteine |
RMSD bond length | 0.013 |
RMSD bond angle | 1.860 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((1.11.1_2575: 000)) |
Refinement software | PHENIX ((1.11.1_2575: 000)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.566 |
Number of reflections | 68453 | 6690 |
<I/σ(I)> | 13.3 | 1.9 |
Completeness [%] | 99.1 | 98.7 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM HEPES, pH 6.8 - 7.6, 10-30% PEG MME 2000, 5 mM 5'-deoxyadenosine, and 10 mM L-methionine |