5VRE

Crystal structure of a lysosomal potassium-selective channel TMEM175 homolog from Chamaesiphon Minutus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	152.660, 108.880, 119.330
Unit cell angles	90.00, 117.24, 90.00

Refinement procedure

Resolution	42.465 - 3.299
R-factor	0.2606
Rwork	0.259
R-free	0.28180
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.299
Rmerge	0.087
Number of reflections	21668
<I/σ(I)>	30.5
Completeness [%]	99.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	16-22% PEG 1000, 50 mM CaCl2, 50 mM MgCl2, 100 mM NaAc pH4.6