5VR8
Human UDP-Glucose Dehydrogenase with UDP-Xylose Bound to the Co-enzyme Site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 89.080, 196.490, 111.260 |
Unit cell angles | 90.00, 111.91, 90.00 |
Refinement procedure
Resolution | 49.123 - 1.999 |
R-factor | 0.1578 |
Rwork | 0.156 |
R-free | 0.18960 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.907 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.123 |
High resolution limit [Å] | 1.999 |
Number of reflections | 222659 |
<I/σ(I)> | 14.91 |
Completeness [%] | 93.2 |
Redundancy | 12.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298.15 | 14% 1-6 Hexanediol, 10% PEG 3350, 100mM HEPEs, 10mM Adenosine Diphosphate, 5mM UDP-Xylose |