5VR4
RNA octamer containing 2'-F-4'-OMe U.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 44.387, 44.387, 85.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.481 - 1.500 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3p4a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.804 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.067 | 0.795 |
Rpim | 0.027 | 0.317 |
Number of reflections | 26152 | |
<I/σ(I)> | 21.05 | 2.73 |
Completeness [%] | 99.6 | 100 |
Redundancy | 7.3 | 7.3 |
CC(1/2) | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 0.5 mM RNA, 10 mM Cobalt(III) hexamine, 6 mM sodium chloride, 40 mM potassium chloride, 5% MPD, 20 mM sodium cacodylate equilibrated against reservoir containing 40% MPD |