5VPF

Transcription factor FosB/JunD bZIP domain in complex with cognate DNA, type-II crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	119.637, 119.637, 249.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.593 - 2.694
R-factor	0.2129
Rwork	0.210
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fos
RMSD bond length	0.006
RMSD bond angle	0.792
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.107	0.080	1.752
Rmeas	0.111	0.084	1.813
Rpim	0.029	0.024	0.462
Total number of observations	473877
Number of reflections	30141
<I/σ(I)>	8.8
Completeness [%]	99.9	99.2	100
Redundancy	15.7	14.1	15.5
CC(1/2)		0.971	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.9 M Na2HPO4