5VPF
Transcription factor FosB/JunD bZIP domain in complex with cognate DNA, type-II crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 119.637, 119.637, 249.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.593 - 2.694 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fos |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.792 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.107 | 0.080 | 1.752 |
| Rmeas | 0.111 | 0.084 | 1.813 |
| Rpim | 0.029 | 0.024 | 0.462 |
| Total number of observations | 473877 | ||
| Number of reflections | 30141 | ||
| <I/σ(I)> | 8.8 | ||
| Completeness [%] | 99.9 | 99.2 | 100 |
| Redundancy | 15.7 | 14.1 | 15.5 |
| CC(1/2) | 0.971 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.9 M Na2HPO4 |
