5VP8
I38T mutant of 2009 H1N1 PA Endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 90.098, 90.098, 134.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.190 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5czn |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.367 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.087 | 0.055 | 0.919 |
| Rmeas | 0.090 | 0.057 | 0.978 |
| Rpim | 0.025 | 0.016 | 0.317 |
| Total number of observations | 178503 | ||
| Number of reflections | 14303 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 99.3 | 99.6 | 94.6 |
| Redundancy | 12.5 | 12.2 | 8.3 |
| CC(1/2) | 0.998 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M HEPES pH 7.8, 1 M Ammonium Sulfate, 10 mM MnCl2, 10 mM MgCl2, 0.5% PVP K15 |
