5VOE
DesGla-XaS195A Bound to Aptamer 11F7t
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.916, 164.596, 124.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.300 - 2.000 |
| R-factor | 0.21476 |
| Rwork | 0.213 |
| R-free | 0.24841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y5g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.137 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.300 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.057 | 1.204 |
| Number of reflections | 38141 | 3725 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Bicine, 0.1 M Tris base, 0.1 M Carboxylic Acids, 10% PEG 20,000, 20% PEG 500 monomethyl ester |
