5VO0
Structure of a TRAF6-Ubc13~Ub complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 181.114, 181.114, 97.414 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 128.400 - 3.900 |
R-factor | 0.25468 |
Rwork | 0.252 |
R-free | 0.29920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hct |
RMSD bond length | 0.009 |
RMSD bond angle | 1.731 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 128.400 | 49.370 | 4.360 |
High resolution limit [Å] | 3.900 | 8.720 | 3.900 |
Rmerge | 0.186 | 0.046 | 0.701 |
Rmeas | 0.208 | 0.052 | 0.782 |
Rpim | 0.091 | 0.023 | 0.340 |
Number of reflections | 14890 | ||
<I/σ(I)> | 7.9 | ||
Completeness [%] | 97.9 | 95.8 | 98.6 |
Redundancy | 4.9 | 4.6 | 4.9 |
CC(1/2) | 0.993 | 0.997 | 0.802 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 100-200 mM Na/K tartrate, 11-15% PEG 3350 and 100 mM bis-Tris propane pH 7.5 |