5VNO
Crystal structure of Sec23a/Sec24a/Sec22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-07-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.046, 96.727, 126.796 |
Unit cell angles | 90.00, 91.45, 90.00 |
Refinement procedure
Resolution | 49.940 - 2.905 |
R-factor | 0.2464 |
Rwork | 0.244 |
R-free | 0.29840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nut |
RMSD bond length | 0.005 |
RMSD bond angle | 0.595 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 42151 |
<I/σ(I)> | 15.9 |
Completeness [%] | 98.6 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50mM HEPES PH 7.8, 0.5M NaCl and 12% PEG4K |