5VNL
Crystal structure of Sec23a/Sec24a/Sec22 complexed with 4-phenylbutyric acid (1mM soaking)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.756, 96.770, 129.590 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 48.776 - 2.394 |
| R-factor | 0.2092 |
| Rwork | 0.208 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nut |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.645 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.776 |
| High resolution limit [Å] | 2.394 |
| Number of reflections | 61011 |
| <I/σ(I)> | 1.2 |
| Completeness [%] | 98.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K |
