5VNK

Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal LL sorting motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-07-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	148.180, 97.615, 130.546
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	130.546 - 2.550
R-factor	0.1987
Rwork	0.196
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nut
RMSD bond length	0.003
RMSD bond angle	0.645
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	130.546
High resolution limit [Å]	2.550
Number of reflections	60273
<I/σ(I)>	2.3
Completeness [%]	99.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K