5VNJ
Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal FF sorting motif (ERGIC-53)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2015-07-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.955, 96.978, 129.549 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 48.826 - 2.806 |
| R-factor | 0.2226 |
| Rwork | 0.221 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nut |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.499 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.826 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 44706 |
| <I/σ(I)> | 1 |
| Completeness [%] | 98.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K |
