5VNH
Crystal structure of Sec23a/Sec24a/Sec22 complexed with a C-terminal SV sorting motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-07-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.937, 96.895, 127.341 |
Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
Resolution | 48.881 - 2.600 |
R-factor | 0.2129 |
Rwork | 0.212 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nut |
RMSD bond length | 0.002 |
RMSD bond angle | 0.541 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.881 |
High resolution limit [Å] | 2.600 |
Number of reflections | 61690 |
<I/σ(I)> | 2.5 |
Completeness [%] | 99.5 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50mM HEPES PH 7.8, 0.5M NaAc and 12% PEG4K |